Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.
نویسندگان
چکیده
The development of a microtiter solid-phase radioimmunoassay for the detection of Vibrio cholerae enterotoxin and heat-labile Escherichia coli enterotoxin is described. The test is based on the immunological similarity between V. cholerae toxin and E. coli heat-labile toxin. The assay is easy to perform, quantitative, and at least as sensitive and specific as the Y-1 adrenal cell system.
منابع مشابه
Solid-phase microtiter radioimmunoassay blocking test for detection of antibodies to Escherichia coli heat-labile enterotoxin.
The development of a solid-phase microtiter radioimmunoassay blocking test to detect serum antibody to Escherichia coli heat-labile enterotoxin is described. The assay is easy to perform and quantitate, and it is sensitive and specific.
متن کاملEnzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA s...
متن کاملConstruction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated Form of the Heat-Stable Enterotoxins in Escherichia coli
Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
متن کاملModified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.
The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results correlated well with those of the Chinese hamster ovary cell assay and passive immune hemolysis. It is concluded that the modified Elek test is a simple and reproducible assay method for identificati...
متن کاملAn LTB-entrapped protein in PLGA nanoparticles preserves against enterotoxin of enterotoxigenic Escherichia coli
Objective(s): Enterotoxigenic Escherichia coli (ETEC) is known as the most common bacterial causes of diarrheal diseases related to morbidity and mortality. Heat-labile enterotoxin (LT) is a part of major virulence factors in ETEC pathogenesis. Antigen entrapment into nanoparticles (NPs) can protect them and enhance their immunogenicity.Materials and Methods: In the present study, recombinant L...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Infection and immunity
دوره 17 3 شماره
صفحات -
تاریخ انتشار 1977